PIK3CA gene mutations in Northwest Chinese esophageal squamous cell carcinoma

Table 2 Primers of PIK3CA gene for pyrosequencing

Exon	Primers
Exon 9 RS1	Nucleotide dispensation order	5’ CCATAGAAAATCTTTCTCCT 3’
		5’ ATCGACTACACTGACTGACTGACTGACTGACTGACTG 3’
Exon 9 RS2	Nucleotide dispensation order	5’ TTCTCCTTGCTTCAGTGATTT 3’
		5’ ATACACATGTCAGTCAGACTAGCTAGCTAGCTAG 3’
Exon 9 RS3	Nucleotide dispensation order	5’ TAGAAAATCTTTCTCCTGCT 3’
		5’ ATAGCACTGACTGACTGACTACTGACTGACTGACTG 3’
Exon 20 RS	Nucleotide dispensation order	5’TGGAATGCCAGAACTACAATCTTT 3’
		5’GGTCTTTGCCTGCTGAGAGTT 3’