MicroRNA-145 exerts tumor-suppressive and chemo-resistance lowering effects by targeting CD44 in gastric cancer

Figure 3 Overexpression of CD44 abolished the inhibitory effect of miR-145 on tumor sphere formation in gastric cancer cells. ^eP < 0.001 vs cells transfected with the control; ^bP < 0.01 vs cells transfected with miR-145 mimics; data are the mean ± SEM of at least three independent experiments. A: Transfection with plasmid pLV-CD44 increased CD44 protein expression significantly; B: CD44 overexpression abolished the inhibitory effect of miR-145 on tumor sphere formation. MGC-803 cells were co-transfected with pLV-CD44 with or without miR-145 mimics, respectively, and the transfected cells were collected 12 h later for tumor sphere formation assays as described.