CMA down-regulates p53 expression through degradation of HMGB1 protein to inhibit irradiation-triggered apoptosis in hepatocellular carcinoma

Figure 4 HMGB1 was degraded through chaperone-mediated autophagy pathway in hepatocellular carcinoma cells. sh-Lamp-2a HepG2 and sh-Lamp-2a SMMC7721 cells were irradiated at doses of 6 Gy, (A) the levels of HMGB1 were determined after 48 h by western blot and (B) the interaction of Lamp-2a with HMGB1 was detected by immunoprecipitation (IP); C: Lamp-2a and HMGB1 were detected at different post-irradiation times by IP; D: In the presence of EP (10 μg/mL), the levels of HMGB1 were detected after 48 h by IP. Each experiment was repeated three times and similar results were obtained.