CMA down-regulates p53 expression through degradation of HMGB1 protein to inhibit irradiation-triggered apoptosis in hepatocellular carcinoma

Figure 2 p53 was regulated through chaperone-mediated autophagy pathway activation in hepatocellular carcinoma cells on irradiation. A: HepG2 and SMMC7721 cells were irradiated with doses of 6 Gy. At different post-irradiation times, the levels of p53 were determined by western blot; B: HepG2 and Hep3B (p53-/-) cells were irradiated at different doses and the ability of proliferation was detected by clone formation assay; C: HepG2 and Hep 3B (p53-/-) cells were irradiated at doses of 6 Gy; the levels of Caspase 3 (cleaved) and Bcl-2 were detected at 48 h by western blot; D: sh-Lamp-2a HepG2 and sh-Lamp-2a SMMC7721 cells were irradiated at doses of 6 Gy; the levels of p53 and p21 were determined after 48 h. ^aP < 0.05 vs control group or sh-NC group; ^cP < 0.05 vs HepG2 groups. Each experiment was repeated three times and similar results were obtained.