CMA down-regulates p53 expression through degradation of HMGB1 protein to inhibit irradiation-triggered apoptosis in hepatocellular carcinoma

Figure 1 Irradiation-induced chaperone-mediated autophagy pathway activation regulated the apoptosis in hepatocellular carcinoma cells. A: HepG2 and SMMC7721 cells were irradiated at different doses and the ability of proliferation was detected by clone formation assay; B: HepG2 and SMMC7721 cells were irradiated at doses of 6 Gy; the levels of Lamp-2a, Caspase 3 (cleaved) and Bcl-2 were determined by western blot at different post-irradiation times; C: sh-Lamp-2a HepG2 and sh-Lamp-2a SMMC7721 cells were irradiated at doses of 6 Gy; the levels of Lamp-2a, Caspase 3 (cleaved) and Bcl-2 were detected at 48 h by western blot; D: The ability of propagation in sh-Lamp-2a HepG2 and sh-Lamp-2a SMMC7721 cells was detected by clone formation assay at 48 h after 6 Gy irradiation. ^aP < 0.05, vs control groups or sh-NC groups. Each experiment was repeated three times and similar results were obtained.