Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice

Figure 7 Recombinant IL-33 and IL-33 from 5-FU-treated ileum induced IL-13Rα2 expression in mouse embryonic fibroblasts. A: Mouse embryonic fibroblasts (MEFs) obtained from WT and Fn14 KO mice were cultured with IL-13 or IL-33 for 3 d and probed with anti-IL-13Rα2 antibody. Green, IL-13Rα2; red, nuclei. Scale bar = 200 µm. IL-13Rα2-positive area was normalized by number of nuclei in the image and quantified (right). Data are shown as mean ± SD of four images from four independent cultures. ^aP < 0.05; B: WT MEFs were cultured with the homogenate of ileum obtained from WT or Fn14 KO mice 2 d after 5-FU treatment. IL-33 was depleted using immunoprecipitation with anti-IL-33 antibody or control IgG. After 3 d of culture with homogenate, cells were probed with anti-IL-13Rα2 antibody. Green, IL-13Rα2; red, nuclei. Scale bar = 100 µm; C: The percent of IL-13Rα2-positive cells was quantified from the images collected during the experiment described in (B). Each dot represents an individual culture. Bars indicate means. ^aP < 0.05. KO indicates Fn14 KO mice.