Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice

Figure 2 Blocking the TWEAK/Fn14 pathway prevents 5-FU-induced diarrhea in tumor-bearing mice. A: CT26 cells were cultured with various doses of anti-TWEAK antibody. Cell numbers were counted at the indicated time using the trypan-blue dye exclusion method. Triplicate cultures were performed for each condition. Data are shown as a mean ± SD; B: CT26 cells were inoculated in WT mice on day -7 (n = 3 per group). Mice were injected with anti-TWEAK antibody or control IgG from day -3, and injections were repeated every other for the remainder of the study period (indicated as arrows). 5-FU was not administered. C26 tumor volume was monitored. Data are shown as mean ± SD; C: Diarrhea score of CT26 tumor-bearing WT mice treated with anti-TWEAK antibody (n = 8) or control IgG (n = 9). Experiment was performed as described in panel B with the addition of a single injection of 5-FU on day 0 (blank arrowhead); D: Tumor volume of measurements from the experiment described in (C).