Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model

Figure 3 Protein expression of phospho-IκBα, nuclear factor-kappa B p65, myosin light chain kinase and phospho-MLC2 (A) and MLCK localization (B) in ileum epithelium. A: Protein expression of phospho-IκBα (A1), nuclear factor-kappa B (NF-κB) p65 (A2), myosin light chain kinase (MLCK) (A3) and phospho-MLC2 (A4) were analyzed by western blotting at 6 h after lipopolysaccharide (LPS) induction, and β-actin was used as internal control (n = 3). Data are shown as mean ± SE (n = 3). ^aP < 0.05, vs the control group; ^cP < 0.05, vs the LPS group; B: MLCK location was observed by immunofluorescence at 6 h after LPS administration at 200 × magnification (red fluorescence) (n = 3). Nuclei were stained by DAPI (blue fluorescence).