Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model

Figure 2 Localization and expression of tight junction proteins, and tight junction proteins ultrastructure in ileum were evaluated 6 h after lipopolysaccharide administration in mice of four groups. A: Effects of Acanthopanax senticosus polysaccharides (ASPS) on distribution of occludin and ZO-1. Staining of both proteins along the villous epithelium at a 200 × magnification (red fluorescence) were observed by immunofluorescence. Nuclei were stained by DAPI (blue fluorescence). Arrows indicate the location of tight junction (TJ) proteins staining; B: Effects of ASPS on intestinal TJ proteins expression of occludin and ZO-1 (n = 3). Protein samples were analyzed by western blotting, and β-actin was used as an internal control. The values are presented as mean ± SE. ^aP < 0.05, vs the control group; ^cP < 0.05, vs the LPS group; C: Effects of ASPS on intestinal TJ ultrastructure in ileum viewed under transmission electron microscope of C1, control group; C2, LPS group; C3, ASPS + LPS group; and C4, LPS + ASPS group. Arrows indicate the location of the TJ (scale bar = 1 μm).