Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model

Figure 1 Effects of acanthopanax senticosus polysaccharides on clinical score, macroscopic features of distal ileum and colon, histological appearance and score of distal ileum in lipopolysaccharide-induced mice. A: Mice were assessed for clinical score at designated time points after lipopolysaccharide (LPS) challenge (n = 8); B: Representative photographs of the distal ileums and colons at 6 h after LPS injection (n = 8); C: Effects of acanthopanax senticosus polysaccharides (ASPS) on LPS-induced intestinal histopathologic changes. Ileum was processed for morphological and histopathologic evaluation at 6 h after LPS induction (n = 3). The representative photomicrographs of ileal segments stained with hematoxylin and eosin at 200 × magnification of C1, control group; C2, LPS group; C3, ASPS + LPS group; and C4, LPS + ASPS group; D: Intestinal histopathologic score was determined at 6 h after LPS challenge (n = 3); E: Effects of ASPS on LPS-induced increase in iliac mucosal permeability. The intestinal permeability of 4 kDa fluorescein isothiocyanate (FITC)-dextran in ileal pouch was measured at 2, 4 and 6 h after LPS administration (n = 8). ^aP < 0.05, vs the control group; ^cP < 0.05, vs the LPS group.