Integrated analysis of microRNA and mRNA expression profiles in HBx-expressing hepatic cells

Figure 1 Differential mRNA expression between L02/HBx and L02/pcDNA cells. A: The global expression profile of L02/HBx and L02/pcDNA cells was analyzed by RNA-sequencing analysis. Heat map shows the differential gene expression patterns (fold-change > 2 or P < 0.5); B: The 20 top-ranking upregulated (left) and downregulated (right) genes represented on a heat map; C: Quantitative real-time PCR (Q-PCR) verification of selected differential gene expression in L02/HBx and L02/pcDNA cells. The relative expression levels of these genes were normalized to GAPDH; D: Correlation analysis of mRNA expression data detected by microarray and Q-PCR respectively (P = 0.0369). ^aP < 0.05.