Role of CD56-expressing immature biliary epithelial cells in biliary atresia

Figure 3 Immunohistochemical analysis of the expression of cytokeratin 7, epithelial cell adhesion molecule, and CD56. Tissue sections from patients with BA (A-C), NH (D-F), CC (G-I), and the disease control (J-L) together with a set of adjacent sections (M-O) were selected. Immunohistochemical staining was performed with antibodies for CK7 (A, D, G, J and M), EpCAM (B, E, H, K and N) and CD56 (C, F, I, L and O). After antigen retrieval, the sections were incubated with antibodies overnight at 37  °C. The black arrow in (H) indicates a bile duct. The sections were examined with a Nikon light microscope. Scale bar represents 50 μm. EpCAM: Epithelial cell adhesion molecule; CK7: Cytokeratin 7; NH: Neonatal hepatitis; CC: Choledochal cyst; BA: Biliary atresia.