Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation

Figure 5 Effect of different fatty acids on nuclear factor-kappa B/peroxisome proliferator-activated receptor-γ expression. Primary hepatocytes were isolated and treated with PA (0.5 mmol/L), DHA (50 μmol/L) or LPS (1 μg/mL) alone or both of them. (A) mRNA expression of PPAR-γ, (B) Protein expression of NF-κB and PPAR-γ. Values are mean ± SEM, ^aP < 0.05, ^bP < 0.01 vs control, n = 3 experiments. PPAR-γ: Peroxisome proliferator-activated receptor-γ; NF-κB: Nuclear factor-kappa B; PA: Palmitic acid; DHA: Docosahexaenoic acid; LPS: Lipopolysaccharide.