Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation

Figure 4 Effect of different fatty acid diets combined with lipopolysaccharide on hepatic NOD-like receptor protein 3 inflammasome in vivo. Wild-type C57BL/6 mice were fed either normal control (NC) diet, high-fat diet (HFD) or PUFA-enriched diet (PUFA) for 4 wk and then injected with LPS (1.5 mg/kg) before being sacrificed. Liver tissues were obtained for the following analysis. A: mRNA expression of NLRP3 inflammasome components; B: Protein expression of NLRP3 and caspase-1; C: Caspase-1 activity; and D: Mature IL-1β secretion in liver. Values are mean ± SEM, ^aP < 0.05, ^bP < 0.01 vs NC + LPS, n = 5 animals per group. NLRP3: NOD-like receptor protein 3; GAPDH: Reduced glyceraldehyde-phosphate dehydrogenase; LPS: Lipopolysaccharide; IL-1β: Interleukine-1 beta.