Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation

Figure 2 Effect of different fatty acids on NOD-like receptor protein 3 inflammasome activation in hepatocytes. Primary hepatocytes were isolated from wild-type C57BL/6 mice and were treated with either palmitic acid (PA, 0.5 mmol/L) or docosahexaenoic acid (DHA, 50 μmol/L). A: Lipid deposition in hepatocytes after exposure to PA or DHA for 24 h; B: mRNA expression of NLRP3 inflammasome components after FFAs treatment for 6 h; C: Protein expression of NLRP3 and caspase-1 in Western blot; D: Caspase-1 activity and E: Culture supernatant IL-1β level. Values are Mean ± SEM, ^aP < 0.05, ^bP < 0.01 vs normal control (NC), n = 3 experiments. NLRP3: NOD-like receptor protein 3; GAPDH: Reduced glyceraldehyde-phosphate dehydrogenase; LPS: Lipopolysaccharide; IL-1β: Interleukine-1 beta.