Dietary saturated fatty acid and polyunsaturated fatty acid oppositely affect hepatic NOD-like receptor protein 3 inflammasome through regulating nuclear factor-kappa B activation

Figure 1 High fat diets induce hepatic steatosis and NOD-like receptor protein 3 inflammasome activation. Wild-type C57BL/6 mice were fed either normal control (NC) diet or high-fat diet (HFD) for 14 wk. A: Liver histology (magnification × 100); B: mRNA expression of NLRP3 inflammasome components and proinflammatory cytokines, determined by quantitative real-time PCR; C: Protein expression of NLRP3 and caspase-1 in Western blot; D: Caspase-1 activity in liver tissue; and E: Mature IL-1β secretion in liver. Values are Mean ± SEM, ^aP < 0.05, ^bP < 0.01 vs NC; n = 10 animals per group. NLRP3: NOD-like receptor protein 3; GAPDH: Reduced glyceraldehyde-phosphate dehydrogenase; IL-1β: Interleukine-1 beta.