Regulating effect of TongXie-YaoFang on colonic epithelial secretion via Cl- and HCO3- channel

Figure 2 Effects of 1,1-dimethyl piperidinium chloride, Glibenclamide, cystic fibrosis transmembrane conductance regulator(inh)-172, 4,4’-diisothiocyanato-stilbene-2,2’-disulfonic acid, Amiloride and Bumetanide on 5-HT-induced the changes of short-circuit current in rats. A: After the intervention with DPC, Glibenclamide or CFTR(inh)-172, respectively, ∆Isc induced by 5-HT were similar in three groups (P > 0.05, respectively); B: 5-HT-induced ∆Isc was higher in TXYF-formula group than in NMS + RS group after the intervention with DIDS or Amiloride. ^aP < 0.05 vs NMS + RS group; ^bP < 0.01 vs NMS + RS group. DPC: 1,1-dimethyl piperidinium chloride; CFTR: Cystic fibrosis transmembrane conductance regulator; ∆Isc: the changes short-circuit current; DIDS: 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid; TXYF: TongXie-YaoFang; NMS: Neonatal maternal separation; RS: Restraint stress; NH: No handlings as control.