Helicobacter pylori inhibits the cleavage of TRAF1 via a CagA-dependent mechanism

Figure 3 Helicobacter pylori inhibits the cleavage of tumor necrosis factor receptor-associated factor 1. A: AGS cells transfected with HA-tagged tumor necrosis factor receptor-associated factor 1 (TRAF1) were treated with the apoptosis inducer (0.3 μg/mL CHX and 80 ng/mL TNF-α) and co-cultured with Helicobacter pylori (H. pylori) strain NCTC11637, or left untreated for the infection time indicated, and analyzed for the cleavage of TRAF1 by western blotting; B: AGS cells transfected with HA-tagged TRAF1 were treated with the apoptosis inducer and co-cultured with H. pylori strain NCTC11637, or left untreated, at the indicated cell/bacteria ratios and analyzed for the cleavage of TRAF1 by western blotting; C: The same as shown in (A), but analyzed for the uncleaved and cleaved caspase-8 by western blotting; D: The same as shown in (B), but analyzed for the uncleaved and cleaved caspase-8 by western blotting.