Helicobacter pylori inhibits the cleavage of TRAF1 via a CagA-dependent mechanism

Figure 2 COOH-terminal tumor necrosis factor receptor-associated factor 1 fragment promoted cell apoptosis in Helicobacter pylori infected cells. A: AGS cells were transfected with HA-tagged tumor necrosis factor receptor-associated factor 1 (TRAF1), TRAF1(D163A), TRAF1-N, TRAF1-C for 24 h and then analyzed for cell apoptosis by flow cytometry using annexin V-FITC and propidium iodide staining (^aP < 0.05 vs control). The expression levels of these proteins in the analyzed cells were shown (Lower); B: AGS cells were transfected with HA-tagged TRAF1, TRAF1(D163A), TRAF1-N, TRAF1-C for 24 h respectively, followed by infection with Helicobacter pylori strain NCTC11637 for 24 h and then analyzed for cell apoptosis (^aP < 0.05 vs control, ^bP < 0.01 vs Control, ^cP < 0.05 vs control). The expression levels of these proteins in the analyzed cells are shown (Lower).