Effects of asymmetric dimethylarginine on renal arteries in portal hypertension and cirrhosis

Figure 4 DDAH1 and DDAH2 expression in kidneys from portal hypertensive and cirrhotic rats. A and B: DDAH1 and DDAH2 mRNA expression in kidney from Sham, PPVL, and BDL groups normalized to the expression of GAPDH, which was used as an endogenous reference gene; C and D: Immunoblot analysis in single kidney probed with antibodies against DDAH1, DDAH2 or β-actin, as indicated; E and F: Graphs show the results of densitometric analyses from pooled data, plotted as optical densitometry relative to the signal obtained by β-actin. Each data set represents the mean ± SEM derived from 6 independent experiments. ^aP < 0.05 vs Sham group and ^cP < 0.05 vs PPVL group. DDAH: Dimethylarginine dimethylaminohydrolase.