Vitamin D differentially regulates Salmonella-induced intestine epithelial autophagy and interleukin-1β expression

Figure 1 Effect of 1,25D3 on autophagy expression in Salmonella-infected Caco-2 cells. Caco-2 cells were untreated (CON) or treated with 1,25D3 (D3) and then infected by S. typhimurium wild-type strain SL1344 (SL). Immunoblots were performed on whole cell lysates with antibody to detect autophagy LC3-I/II protein expression. The results shown are representative of three separate experiments. GAPDH was detected for normalization of cytosolic protein. Representative immunoblots (A) and densitometric quantification of immunoreactive bands (B) are shown. The relative band intensities of LC3-II in untreated (white) and treated (black) Caco-2 cells were quantified as fold-increases compared with the control cells.