Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma

Table 6 Whole promoter methylation status of genes with inverse age-related CpG site DNA methylation and gene expression data

Gene symbol	DNA methylation on age-related CpG site			Whole promoter DNA methylation status
	cg ID	P value	Δβ	Start-Stop position	P value	Δβ
CRC vs N
AKT3	cg11314684	4.55 × 10-5	-0.16	chr1:244005801-244005900	6.56 × 10-3	-0.33
ERG	cg17274064	1.48 × 10-4	-0.11	chr21:39871501-39871600	0.044	-0.21
MGP	cg00431549	0.020	-0.12	chr12:15038501-15038600	0.030	-0.27
CCR10	cg09509673	1.91 × 10-4	0.11	chr17:40835101-40835200	0.045	0.18
				chr17:40835201-40835300	0.022	0.26
PPP1R16B	cg24834740	3.56 × 10-12	0.37	chr20:37433201-37433300	3.76 × 10-3	0.35
				chr20:37434701-37434800	0.048	0.40
				chr20:37435301-37435400	0.042	0.37
SFRP1	cg02388150	3.33 × 10-3	0.10	chr8:41166001-41166100	0.017	0.44
				chr8:41166101-41166200	1.31 × 10-4	0.56
				chr8:41166201-41166300	3.40 × 10-3	0.49
				chr8:41166301-41166400	0.014	0.27
				chr8:41166401-41166500	1.28 × 10-3	0.53
				chr8:41166501-41166600	0.027	0.44
				chr8:41166601-41166700	0.034	0.39
				chr8:41166901-41167000	0.042	0.41
SYNE1	cg26620959	1.32 × 10-6	0.30	chr6:152622201-152622300	0.027	0.35
				chr6:152957601-152957700	0.032	0.39
				chr6:152957701-152957800	0.043	0.40
				chr6:152958101-152958200	2.86 × 10-4	0.65
AD vs N
CEMIP	cg20828084	1.33 × 10-9	-0.17	chr15: 81070701-81070800	0.028	-0.25
SPATA18	cg03103192	1.39 × 10-7	-0.15	chr4:52915901-52916000	0.042	-0.23
DKK3	cg13216057	9.64 × 10-4	0.18	chr11:12029701-12029800	3.11 × 10-3	0.26
				chr11:12029801-12029900	1.67 × 10-3	0.33
				chr11:12029901-12030000	8.08 × 10-5	0.56
				chr11:12030001-12030100	0.047	0.15
				chr11:12030401-12030500	7.38 × 10-3	0.28
				chr11: 12030501-12030600	0.012	0.28
PPP1R16B	cg24834740	2.23 × 10-9	0.36	chr20:37434201-37434300	0.045	0.26
				chr20:37434601-37434700	0.022	0.39
				chr20:37434701-37434800	0.013	0.39
				chr20:37434801-37434900	6.87 × 10-3	0.30
				chr20:37434901-37435000	1.70 × 10-3	0.44
				chr20:37435001-37435100	1.93 × 10-3	0.45
				chr20:37435101-37435200	6.49 × 10-3	0.35
				chr20:37435201-37435300	8.93 × 10-4	0.51
				chr20:37435301-37435400	0.014	0.42
SDC2	cg25070637	1.82 × 10-5	0.30	chr8:97505901-97506000	2.30 × 10-3	0.59
				chr8:97506101-97506200	1.96 × 10-4	0.65
				chr8:97506201-97506300	8.43 × 10-4	0.63
				chr8:97506301-97506400	0.012	0.49
				chr8:97506401-97506500	0.019	0.47
				chr8:97506601-97506700	3.99 × 10-3	0.51
				chr8:97506701-97506800	0.021	0.45
				chr8:97506801-97506900	0.014	0.52
				chr8:97507101-97507200	0.015	0.44
				chr8:97507201-97507300	0.010	0.52
				chr8:97507301-97507400	3.47 × 10-3	0.59
SFRP1	cg02388150	1.20 × 10-7	0.15	chr8:41165901-41166000	2.30 × 10-3	0.59
				chr8:41166001-41166100	1.57 × 10-3	0.59
				chr8:41166101-41166200	3.54 × 10-4	0.56
				chr8:41166201-41166300	1.49 × 10-4	0.60
				chr8:41166301-41166400	6.54 × 10-3	0.27
				chr8:41166401-41166500	1.32 × 10-5	0.55
				chr8: 41166501-41166600	2.30 × 10-3	0.60
				chr8:41166601-41166700	5.45 × 10-3	0.57
				chr8:41166701-41166800	8.15 × 10-3	0.51
				chr8:41166801-41166900	0.032	0.39
				chr8:41166901-41167000	0.019	0.45
				chr8:41167001-41167100	0.046	0.34
				chr8:41167101-41167200	0.025	0.41
				chr8:41167201-41167300	0.024	0.40
SYNE1	cg26620959	3.89 × 10-6	0.29	chr6:152702701-152702800	0.012	0.20
				chr6:152702801-152702900	0.035	0.21
				chr6:152957501-152957600	6.05 × 10-4	0.37
				chr6:152957601-152957700	2.34 × 10-4	0.60
				chr6:152957701-152957800	0.017	0.51
				chr6:152957801-152957900	1.09 × 10-3	0.42
				chr6:152957901-152958000	1.03 × 10-4	0.57
				chr6:152958001-152958100	7.13 × 10-4	0.43
				chr6:152958101-152958200	1.72 × 10-5	0.72
CRC vs AD
CEMIP	cg20828084	2.00 × 10-8	0.14	chr15:81070701-81070800	7.59 × 10-4	0.31
				chr15:81070801-81070900	0.011	0.36

Only significant promoter DNA methylation alterations are represented in the Table. P < 0.05.