Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma

Table 5 Genes showing inverse DNA methylation and gene expression data

Gene symbol	Gene name	DNA methylation			Gene expression
		cg ID	P value	Δβ	Affymetrix ID	P value	LogFC
CRC vs N
AKT3	AKT serine/threonine kinase 3	cg11314684	4.55 × 10-5	-0.16	224229_s_at	0.015	0.07
					222880_at	2.26 × 10-3	0.61
					212609_s_at	4.72 × 10-4	0.62
					212607_at	9.76 × 10-4	0.64
ERG	v-ets avian erythroblastosis virus E26 oncogene homolog	cg17274064	1.48 × 10-4	-0.11	211626_x_at	5.84 × 10-4	0.20
					241926_s_at	2.05 × 10-5	0.64
					213541_s_at	4.69 × 10-9	1.08
MGP	Matrix Gla protein	cg00431549	0.020	-0.12	202291_s_at	2.34 × 10-11	1.97
PIPOX	Pipecolic acid and sarcosine oxidase	cg06144905	1.81 × 10-7	-0.22	221605_s_at	0.019	0.50
CCR10	C-C motif chemokine receptor 10	cg09509673	1.91 × 10-4	0.11	220565_at	0.010	-0.51
CLEC3B	C-type lectin domain family 3 member B	cg06117855	1.88 × 10-3	0.11	205200_at	1.45 × 10-13	-1.79
LTK	Leukocyte receptor tyrosine kinase	cg27494383	9.32 × 10-3	0.12	217184_s_at	2.14 × 10-11	-1.32
					207106_s_at	2.00 × 10-5	-0.75
PPP1R16B	Protein phosphatase 1 regulatory subunit 16B	cg24834740	3.56 × 10-12	0.37	41577_at	1.76 × 10-4	-0.79
					212750_at	7.68 × 10-3	-0.48
PRKAA2	Protein kinase AMP-activated catalytic subunit alpha 2	cg06836772	0.013	0.14	227892_at	4.15 × 10-3	-0.87
					238489_at	4.67 × 10-3	-0.22
SFRP1	Secreted frizzled related protein 1	cg02388150	3.33 × 10-3	0.10	202036_s_at	8.66 × 10-8	-1.47
					202037_s_at	3.98 × 10-5	-1.11
					202035_s_at	0.039	-0.28
SYNE1	Spectrin repeat containing nuclear envelope protein 1	cg26620959	1.32 × 10-6	0.30	209447_at	4.27 × 10-5	-0.63
AD vs N
CEMIP	Cell migration inducing hyaluronan binding protein	cg20828084	1.33 × 10-9	-0.17	1554685_a_at	1.71 × 10-11	1.12
					212942_s_at	2.41 × 10-43	6.88
NT5DC3	5'-nucleotidase domain containing 3	cg06462291	7.45 × 10-5	-0.25	218786_at	6.83 × 10-9	0.76
SPATA18	Spermatogenesis associated 18	cg03103192	1.39 × 10-7	-0.15	230723_at	4.41 × 10-5	0.45
					229331_at	1.20 × 10-10	1.50
DKK3	Dickkopf WNT signaling pathway inhibitor 3	cg13216057	9.64 × 10-4	0.18	202196_s_at	9.82 × 10-5	-0.65
					214247_s_at	1.52 × 10-4	-0.50
PPP1R16B	Protein phosphatase 1 regulatory subunit 16B	cg24834740	2.23 × 10-9	0.36	41577_at	1.20 × 10-10	-1.44
					212750_at	3.28 × 10-7	-0.81
SDC2	Syndecan 2	cg25070637	1.82 × 10-5	0.30	212158_at	1.74 × 10-10	-1.17
					212157_at	2.14 × 10-8	-0.66
					212154_at	9.19 × 10-5	-0.64
SFRP1	Secreted frizzled related protein 1	cg02388150	1.20 × 10-7	0.15	202036_s_at	3.62 × 10-13	-1.79
					202037_s_at	4.43 × 10-11	-1.48
					202035_s_at	0.016	-0.32
SYNE1	Spectrin repeat containing nuclear envelope protein 1	cg26620959	3.89 × 10-6	0.29	209447_at	5.76 × 10-15	-1.19
CRC vs AD
CEMIP	Cell migration inducing hyaluronan binding protein	cg20828084	2.00 × 10-8	0.14	212942_s_at	9.88 × 10-3	-1.17
SLC28A2	Solute carrier family 28 member 2	cg19305227	1.11 × 10-6	0.14	207249_s_at	0.027	-1.24
SPATA18	Spermatogenesis associated 18	cg03103192	6.07 × 10-12	0.16	229331_at	3.52 × 10-9	-1.73
					230723_at	7.35 × 10-4	-0.41

N: Normal; Ad: Adenoma; CRC: Colorectal cancer.