C5a/C5aR pathway is essential for up-regulating SphK1 expression through p38-MAPK activation in acute liver failure

Figure 4 C5a/C5aR signaling is required for the expression of SphK1 during acute liver failure. A and B: Blocking of C5aR with C5aRa reduced SphK1 expression in liver tissue and peripheral blood mononuclear cells (PBMCs) of acute liver failure (ALF) mice; C: Blocking of C5aR by C5aRa reduced S1P level in liver tissue significantly than that of control (ALF group), ^eP < 0.01; D, E: Recombinant murine C5a (500 ng/mL) induced SphK1 expression in peritoneal exudative macrophages (PEMs) and RAW 264.7 cells, and was abolished by incubation for 60 min with 10 nmol/L C5aRa. The data shown are representative of three separate experiments.