CYP1A1, CYP2E1 and EPHX1 polymorphisms in sporadic colorectal neoplasms

Table 2 Description of the primers sequences and restriction enzymes for CYP1A1*2A, CYP2E1*5B and CYP2E1*6 polymorphisms analysis

Polymorphisms	Sequence of primers	Restriction Enzyme
		T/t
CYP1A1*2A		MspI
Sense	5’-GA TGA AGA GGT GTA GCC GCT-3’	37 °C/3 h
Antisense	5-TAG GAG TCT TGT CTC ATG CCT-3’
CYP2E1*5B		PstI
Sense	5’-CCA GTC GAG TCT ACA TTG TCA-3’	37 °C/3 h
Antisense	5’-TTC ATT CTG TCT TCT AAC TGG-3’
CYP2E1*6		DraI
Sense	5’-TCG TCA GTT CCT GAA AGC AGG-3’	37 °C/3 h
Antisense	5’-GAG CTC TGA TGC AAG TAT CGC-3’