Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer

Figure 2 Anticancer mechanism of linalool. A: TUNEL staining of HCT 116 cells in the control group; B: Staining of HCT 116 cells treated with 100 μmol/L linalool; C: Staining of HCT 116 cells treated with 50 nmol/L staurosporine. The corresponding percentage of TUNEL-positive cells (white arrow) was 0.7% ± 0.7% (A), 10.5% ± 7.7% (B), and 25.1% ± 11.2% (C); D: The percentage of apoptotic cells in each group (mean ± SD, n = 10). ^aP < 0.05 vs control group; ^bP < 0.05 vs 100 μmol/L linalool group; E: Flow cytometry using Annexin V for the detection of live cells, cells showing early and late apoptosis, and cell death; F: Percentages of early and late apoptosis fraction. ^cP < 0.05 vs control group; ^dP < 0.01 vs control group; ^eP < 0.05 vs control group; one-way ANOVA followed by Dunnett’s test, n = 3.