Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

Figure 3 Double-immunostaining with TRPV1 and GFAP or PGP9. 5 of large intestine longitudinal muscle layer-myenteric plexus of wild-type mice. LM-MP was isolated from 5-wk-old mice and whole-mount immunohistochemistry was performed as described in Materials and Methods. TRPV1 stained both cell bodies and fibers, while GFAP stained mainly fibers and some portions of their IR signals were co-localized. Co-localization of PGP9.5 and TRPV1-IR signals was not observed. Nuclei were stained with DAPI. Representative data from 2 experiments using 2 mice per time point are shown. Scale bar represents 50 μm. TRPV1: Transient receptor potential vanilloid 1; GFAP: Glial fibrillary acidic protein; LM-MP: Longitudinal muscle layer-myenteric plexus; WT: Wild-type.