Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

Figure 1 Double-immunostaining with GFAP and TRPV1 of small and large intestines of wild-type and transient receptor potential vanilloid 1-deficient mice. The degree of GFAP-IR signal observed in the enteric nervous system was similar in WT and KO mice. TRPV1-IR signal was observed only in WT mice and was located in a confined area of the smooth muscle layer. Nuclei were stained with TO-PRO3. Representative data from 2 experiments using 2 mice per time point are shown. Scale bar represents 200 μm. TRPV1: Transient receptor potential vanilloid 1; SI: Small intestine; LI: Large intestine; WT: Wild-type; KO: Knockout; GFAP: Glial fibrillary acidic protein.