Hepatitis B virus upregulates host expression of α-1,2-mannosidases via the PPARα pathway

Figure 3 α-1,2-mannosidases are upregulated in the HBs, preS2 and preS1-transfected hepatoma cells. Hepatitis B virus protein expression and effect on MAN1C1 (A) Western blot indicating the HBs, preS2 and preS1 expression in PTT22-HBs, PTT22-preS2, and PTT22-preS1 transfected cells; B: Western blot indicating the HBx expression in PTT22-HBx transfected cells; C: Western blot indicating the HBp expression in PTT22-HBp transfected cells; D: Western blot indicating the HBc expression in PTT22-HBc transfected cells; E: ELISA indicating the HBe expression in PTT22-HBe transfected cells; F: Western blot indicating that MAN1C1 expression is significantly increased in PTT22-HBs, PTT22-preS2, and PTT22-preS1 transfected cells; G: Quantification of MAN1C1 from the band intensities normalized by ACTIN. ^aP < 0.05 vs PTT22 vector controls. The data shown represent the mean ± SD of three independent experiments. HBV: Plasmids (PTT22-HBV) were transfected into HepG2 cells. HBs: Plasmids (PTT22-HBs) were transfected into HepG2 cells. preS2: Plasmids (PTT22-preS2) were transfected into HepG2 cells. preS1: Plasmids (PTT22-HBV) were transfected into HepG2 cells. HBx: Plasmids (PTT22-HBx) were transfected into HepG2 cells. HBp: Plasmids (PTT22- HBp) were transfected into HepG2 cells. HBc: Plasmids (PTT22-HBc) were transfected into HepG2 cells. vector: Plasmids (PTT22-GFP) were transfected into HepG2 cells. LLOQ: Lower limit of quantification.