IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitis in vitro

Figure 4 Reversion of the polarization of lung macrophages in acute pancreatitis group. Lung alveolus macrophages were obtained 24 h after acute pancreatitis (SAP) model establishment and respectively treated with PBS, IL-4 or interferon regulatory factor 5 (IRF5) siRNA for 8 h (S + PBS, S + IL-4, S + IRF5 siRNA group). The M1/M2 phenotypes were detected by real-time polymerase chain reaction. A: mRNA levels of M1 phenotypes in the macrophages; B: mRNA levels of M2 phenotypes in the macrophages. The statistical significance was analyzed in comparison with the CTRL group (S + PBS). Error bars indicate the mean ± SE. ^aP < 0.05, ^cP < 0.01, ^eP < 0.001 vs control. S: Lung alveolus macrophages from the mouse SAP model.