Genetic polymorphism in CD14 gene, a co-receptor of TLR4 associated with non-alcoholic fatty liver disease

Table 2 Restriction fragment length polymorphism conditions

Polymorphic site	Restriction enzyme	Incubation temperature and duration	Genotype and restriction fragment pattern (bp)
	(units used)
TLR4 (Asp299gly)	NcoI	37 °C for 16 h	AA: 249
			AG: 249, 223, 26
			GG: 223, 26
TLR4 (Thr399Ile)	HinfI	37 °C for 8 h	CC: 406
			CT: 406, 377, 29
			TT: 377, 29
TLR2 (Arg753Gln)	AciI	37 °C for 16 h	GG: 252 and 37
			GA: 252, 37, 289
			AA:289
CD14 (-550C/T)	HaeIII	37 °C for 12 h	CC: 23, 236, 109
			CT: 23, 236, 109, 259
			TT: 109, 259
CD14 (-159C/T)	HaeIII	37 °C for 16 h	CC: 141, 154
			CT: 141, 154, 296
			TT: 296