HER2-induced metastasis is mediated by AKT/JNK/EMT signaling pathway in gastric cancer

Figure 4 Combined effects of HER2 silencing and JNK inhibition on metastatic potential. SNU-216 and NCI-N87 cells were infected with a lentivirus containing either control shRNA (shCtrl) or HER2 shRNA (shHER2) followed by treatment with either DMSO or SP600125 (SP). A, D: After treatment with SP for 24 h, protein expressions of pHER2 and pJNK were determined by Western blot; B, E: After treatment with SP for 48 h, cell migration was evaluated by Transwell migration assay; C, F: After treatment with SP for 48 h, invasion was evaluated by cell invasion assay. Results were calculated as percentages relative to control cells. Data are expressed as mean ± SD (n = 4 per each group). ^aP < 0.05 vs control cells.