Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells

Figure 5 Special AT-rich sequence-binding protein 2 binds to regulatory elements of CD133, CD44, MEIS2 and AXIN2 genes. A-D: ChIP was carried out with anti-SATB2 antibody, anti-RNA pol II antibody and anti-IgG antibody. Input DNA and immunoprecipitated DNA by anti-RNA pol II antibody were used as positive controls. Immunoprecipitated DNA by anti-IgG antibody and H₂O were used as negative controls. PCR was used to find out the genomic binding sites for each gene. Red squares under the genomic sequence mean the predicted Satb2-binding loci. Green boxes mean the exons for each genomic sequence. Black arrows mean the translation initiation sites. As expected, SATB2 bound CD133 (A), CD44 (B), MEIS2 (C) and AXIN2 (D) at their regulatory elements.