Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells

Figure 4 Special AT-rich sequence-binding protein 2 knockdown increases the expression of several markers for cancer stem cells in colorectal cancer cells in vitro. A-B: The mRNA expression of a series of stem cell markers was detected in SATB2 overexpressing cells (A) and SATB2 knockdown cells (B) by qRT-PCR; C-D: CD133 expression level in cells with stable SATB2 knockdown was increased when detected by Western blot (C) and immunofluorescence (D). Scale bar is 10 μm. Data shown are mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, vs control.