Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells

Figure 3 Special AT-rich sequence-binding protein 2 knockdown enhances secondary sphere formation of colorectal cancer cells in vitro. A: Self-renewal abilities of CRC cells in which SATB2 was stably knocked down were evaluated by sequential sphere formation assay; B: Spheres formed primarily were isolated into single cells and then equal amounts of cells were cultured to form secondary spheres. More secondary spheres were formed in SATB2 downregulated cells in SW480 and DLD-1. Scale bar is 50 μm. Data shown are mean ± SEM. ^aP < 0.05 vs control.