Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells

Figure 1 Overexpression of special AT-rich sequence-binding protein 2 inhibits the proliferation and migration of colorectal cancer cells in vitro. A, B: Expression levels of SATB2 in SW480 and SW620 cells transfected with pCAG-SATB2 were increased whenever detected by qRT-PCR (A) or Western blot (B); C: The proliferation abilities of cells with SATB2 overexpression were detected to have a decrease in CCK-8 cell proliferation assay. The P-values of time effect, group effect and their interaction effect were all below 0.001 in SW480 and DLD-1 cells; D: Colony formation assay was used to analyze ability of clone formation in SATB2 overexpressing cells. Cells with SATB2 overexpression formed less clones; E: Cell migration capacities of control cells and SATB2 overexpressing cells were compared by detecting the invaded cell numbers in transwell chambers. Fewer invaded cells were found in SATB2 overexpressing cells. Scale bar is 50 μm. Data shown are mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control.