Embryonic liver fordin is involved in glucose glycolysis of hepatic stellate cell by regulating PI3K/Akt signaling

Figure 3 Glycolysis related-genes are upregulated significantly in the HSCs isolated from the fibrotic livers. A, C: The real-time RT-PCR analysis was used to evaluate the expression of the PFKP and PFKM2 hepatic glycolytic enzymes in the fibrotic livers and control mice. The PFKP, pAKT, and PFKM2 expressions of the mRNA level were increased compared with those of the controls (^bP < 0.01 vs control). GAPDH was used as the control; B, D: The Western blot analysis indicated that the PFKP and PFKM2 expression at the protein level was upregulated in the fibrotic livers; E: The real-time RT-PCR analysis evaluated the hepatic glucose transporter Glut 1 expression in the fibrotic livers and control mice. The expression of Glut 1 mRNA was increased compare with that of the controls (^bP < 0.01 vs control); F: The Western blot analysis indicated that the Glut 1 expression at the protein level was upregulated in the fibrotic livers; G: The real-time RT-PCR analysis evaluated the hepatic lactate transporter MCT4 expression in the fibrotic livers and the control mice. The Glut 1 expression at the mRNA level was increased compared with that of the controls (^bP < 0.01 vs control); H: The western blot analysis indicated that the MCT4 expression at the protein level was upregulated in the fibrotic livers; I: The intracellular lactate was evaluated by a lactate assay kit (^bP < 0.01 vs control).