Embryonic liver fordin is involved in glucose glycolysis of hepatic stellate cell by regulating PI3K/Akt signaling

Figure 2 Glycolysis related-genes are upregulated in liver fibrosis. A, C: The real-time RT-PCR analysis evaluated the expression of the hepatic glycolytic enzymes, PFKP and PFKM2 in fibrotic and control mice. The PFKP and PFKM2 expression of the mRNA level was increased compared with that of the control (^aP < 0.05 vs control). GAPDH was used as the control; B, D: Western blot analysis indicated that the PFKP and PFKM2 expression of the protein level was upregulated in the fibrotic livers; E: The real-time RT-PCR analysis evaluated the hepatic glucose transporter, Glut 1, expression in the fibrotic livers and the control mice. The expression of Glut 1 mRNA was increased compared with that of the controls (^aP < 0.05 vs control); F: Western blot analysis indicated that the expression of the Glut 1 at protein level was upregulated in the fibrotic livers; G: Real-time RT-PCR analysis evaluated the MCT4 hepatic lactate transporter expression in the fibrotic livers and the control mice. The expression of the Glut 1 mRNA was increased compared with that of the controls (^aP < 0.05 vs control); H: Western blot analysis indicated that the expression of MCT4 at protein level was upregulated in the fibrotic liver; I: The intracellular lactate was evaluated by a lactate assay kit (^aP < 0.05 vs control).