Prolonged feeding with guanidinoacetate, a methyl group consumer, exacerbates ethanol-induced liver injury

Figure 8 Effect of dietary ethanol and/or guanidinoacetate on peptidase specific activities of the proteasome. Rats were fed the Lieber DeCarli control or ethanol diet with or without 0.36% GAA supplementation. After 6 wk of feeding, liver cytosols were prepared and assayed for the chymotrypsin- (A) and trypsin-like (B) specific activities of the proteasome as detailed in the “MATERIALS AND METHODS” section. The results are mean ± SEM of 8-10 determinations from each group and activities are expressed as nmol AFC generated/mg protein/h. Values not sharing a common subscript letter are statistically different, P < 0.05 vs control. GAA: Guanidinoacetate.