Prolonged feeding with guanidinoacetate, a methyl group consumer, exacerbates ethanol-induced liver injury

Figure 7 Effect of dietary ethanol and/or guanidinoacetate ingestion on serum homocysteine, insulin, non-essential fatty acid and hepatic non-essential fatty acid levels. Rats were fed the Lieber DeCarli control or ethanol diet with or without 0.36% GAA supplementation. After 6 wk of feeding, serum homocysteine (A) insulin (B) NEFA (C) and hepatic NEFA (Dlevels were determined by HPLC or biochemical analysis as detailed in the Materials and Methods section. The data shown are means ± SEM of 5 determinations. Values not sharing a common subscript letter are statistically different, P < 0.05 vs control. GAA: Guanidinoacetate; NEFA: Non-essential fatty acid.