Prolonged feeding with guanidinoacetate, a methyl group consumer, exacerbates ethanol-induced liver injury

Figure 1 Effect of dietary ethanol or/and guanidinoacetate ingestion on hepatic triglycerides and cholesterol levels. Male Wistar rats were fed the control or ethanol Lieber DeCarli diet with or without 0.36% GAA. After 6 wk of feeding, triglyceride (A) and cholesterol (B) content in the liver lipid extract was determined using the diagnostics kit (Thermo Electron Clinical Chemistry, Louisville, CO). The data shown are mean ± SEM of 5 determinations. Values not sharing a common subscript letter are statistically different, P < 0.05 vs control. GAA: Guanidinoacetate.