Pancreatic neuroendocrine tumor and solid-pseudopapillary neoplasm: Key immunohistochemical profiles for differential diagnosis

Table 1 Immunohistochemical procedures

Antigen (Clone)	Manufacturer	Antigen retrieval	Dilution	Incubation
		solution/temperature/time		temperature/time
Synaptophysin (27G12)	Nichirei Biosciences, Tokyo, Japan	TB/105 °C/10 min	Prediluted	RT/30 min
Chromogranin A (polyclonal)	Dako Japan, Tokyo, Japan	CB/115 °C/10 min	1:500	RT/30 min
Pan-cytokeratin (AE1/AE3)	Dako Japan, Tokyo, Japan	TB/105 °C/10 min	1:100	RT/30 min
E-cadherin (36B5)	Thermo Scientific, Yokohama, Japan	TB/121 °C/10 min	1:20	RT/60 min
Progesterone receptor (IE2)	Roche Diagnostics, Tokyo, Japan	CB/115 °C/10 min	Prediluted	RT/30 min
Vimentin (V9)	Dako Japan, Tokyo, Japan	CB/100 °C/15 min	1:100	RT/30 min
α-1-antitrypsin (polyclonal)	Dako Japan, Tokyo, Japan	No retrieval	1:100	RT/30 min
CD10 (56C6)	Leica Microsystems, Tokyo, Japan	TB/105 °C/10 min	1:40	RT/30 min
β-catenin (β-Catenin-1)	Dako Japan, Tokyo, Japan	TB/105 °C/10 min	1:200	RT/30 min
Ki-67 (MIB-1)	Dako Japan, Tokyo, Japan	CB/100 °C/30 min	1:25	4 °C/overnight

TB: Tris-HCl buffer; CB: Citrate buffer; RT: Room temperature.