Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Oct 14, 2016; 22(38): 8528-8539
Published online Oct 14, 2016. doi: 10.3748/wjg.v22.i38.8528
Table 1 Primer sequences used in quantitative real-time polymerase chain reaction for detection of stem cell marker mRNA expression
GeneSequence
CD133-F5’ TTTGTCTTCTATTCTTGGCTTC 3’
CD133-R5’ ACCTTGTCATAATCAATTTTGG 3’
CD44-F5’ GGTTCATAGAAGGGCACGT 3’
CD44-R5’ TGTCTTCGTCTGGGATGG 3’
CD24-F5’ TCAAGTATTTGGGAAGTG 3’
CD24-R5’ GTGTTCTAAATGTGGCTAT 3’
OCT3/4-F5’ CGACCATCTGCCGCTTTGAG 3’
OCT3/4-R5’ CCCCCTGTCCCCCATTCCTA 3’
KLF4-F5’ TGGGTCTTGAGGAAGTGCTG 3’
KLF4-R5’ TGTTTACGGTAGTGCCTGGTC 3’
SOX2-F5’ CACCTACAGCATGTCCTACTC 3’
SOX2-R5’ CATGCTGTTTCTTACTCTCCTC 3’
PRL-1-F5’ GGCAAACTTCGAGTCTCCT 3’
PRL-1-R5’ CCGGTTGATGAATGGCTAA 3’
MEIS2-F5’ GTCCACGAACTGTGCGATAA 3’
MEIS2-R5’ TTCGGAAGGGTACGGATG 3’
AXIN2-F5’ AGCATTTTAATCAACAGCATCTA 3’
AXIN2-R5’ TAACTAAGAATGTGATCCAAGAA 3’
NANOG-F5’ CAACTGGCCGAAGAATAGCA 3’
NANOG-R5’ GCAGGAGAATTTGGCTGGAA 3’
GAPDH-F5’ GGAGCGAGATCCCTCCAAAAT 3’
GAPDH-R5’ GGCTGTTGTCATACTTCTCATGG 3’