Characterisation of colonic dysplasia-like epithelial atypia in murine colitis

Figure 5 Immunohistochemical detection of Ki67 in Winnie distal colonic mucosa. A: Immunostaining of Ki67 in the distal colon of untreated C57BL6 mice. Image representative of Ki67 localisation in the distal colon of the four C57BL6 mice examined; B: Distal colonic Ki67 localisation representative of six C57BL6 mice exposed to three cycles of 1% dextran sulphate sodium (DSS); C: Distal colon of Winnie mouse without exposure to three cycles of DSS. Ki67-labelling in the epithelium is visible apically approximately half the crypt length; D: Ki67 immunolabelling of the Winnie distal colon exposed to three cycles of DSS. Crypt base proliferative zone extends approximately two-thirds of the crypt length. Submucosal gland (arrowhead) displays few positive nuclei. Scale bar represents a distance of 50 μm.