Copyright
©The Author(s) 2016.
World J Gastroenterol. Sep 28, 2016; 22(36): 8178-8186
Published online Sep 28, 2016. doi: 10.3748/wjg.v22.i36.8178
Published online Sep 28, 2016. doi: 10.3748/wjg.v22.i36.8178
Figure 1 Flow diagram of the preparation of isolated human hepatocytes with a modified four-step retrograde perfusion technique.
Buffers: PBE: Perfusion buffer with EDTA; PB: Perfusion buffer; PBD: Perfusion buffer with dispase; PBC: Perfusion buffer with collagenase; WB: Washing buffer; WBD: Washing buffer with DNase.
- Citation: Meng FY, Liu L, Liu J, Li CY, Wang JP, Yang FH, Chen ZS, Zhou P. Hepatocyte isolation from resected benign tissues: Results of a 5-year experience. World J Gastroenterol 2016; 22(36): 8178-8186
- URL: https://www.wjgnet.com/1007-9327/full/v22/i36/8178.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i36.8178