Hepatocyte isolation from resected benign tissues: Results of a 5-year experience

Table 2 Reagents and solutions preparation (prepared for 80 g liver sample)

Resolution	Ingredient	Concentration (g/L)
PB (Perfusion buffer)	Double-distilled water (3000 mL)
	NaCl (27 g)	9
	KCl (1.26 g)	0.42
	NaHCO3 (6.3 g)	2.1
	Glucose (2.7 g)	0.9
	Hepes (14.34 g)	4.78
PBE (Perfusion buffer with EDTA)	PB (1000 mL)
	EDTA (0.37 g)	0.37
PBD (Perfusion buffer with dispase)	PB (500 mL)
	Dispase II (Sigma) (4.2 g)	8.4
PBC (Perfusion buffer with collagenase)	PB (500 mL)
	Collagenase IV (Sigma) (0.25 g)	0.5
	CaCl2•2H2O (0.275 g)	0.55
WB (Washing buffer)	Double-distilled water (2500 mL)
	NaCl (17.5 g)	7
	KCl (1.15 g)	0.46
	CaCl2•2H2O (0.325 g)	0.13
	Hepes (5.95 g)	2.38
	Bovine serum albumin (2.5 g)	1.0
WBD (Washing buffer with DNase)	WB (Washing buffer) (1500 mL)
	MgCl2•6H2O (0.15 g)	0.1
	MgSO4•7H2O (0.15 g)	0.1
	DNase I (Sigma) (0.15 g)	0.1