Different pre-S deletion patterns and their association with hepatitis B virus genotypes

Table 4 Frequencies of different types of pre-S deletions in HBV/B- and HBV/C-infected carriers according to their clinical outcome

Deletion	ASC		P value1	CH		P value1	LC		P value1	NC-HCC		P value1	LC-HCC		P value1
	B (n = 2)	C (n = 2)		B (n = 9)	C (n = 11)		B (n = 8)	C (n = 14)		B (n = 14)	C (n = 4)		B (n = 10)	C (n = 12)
N-terminal half of pre-S1 (aa 1-57)	50.0%	50.0%		22.2%	27.3%		50.0%	50.0%		28.6%	50.0%		60.0%	33.3%
C-terminal half of pre-S1 (aa 58-119)	50.0%	0		88.9%	81.8%		87.5%	71.4%		92.9%	75.0%		80.0%	66.7%
N-terminus of pre-S2 (aa 1-31)	0	100.0%		88.9%	72.7%		62.5%	78.6%		78.6%	75.0%		100.0%	58.3%	0.040
d183 M	50.0%	0		33.3%	36.4%		25.0%	28.6%		28.6%	0		0	8.3%
Epitope Mapping
pS1-T1 (aa 12-48 of pre-S1)	50.0%	0		11.1%	18.2%		50.0%	28.6%		21.4%	25.0%		60.0%	16.7%	0.074
pS1-B1 (aa 12-53 of pre-S1)	50.0%	0		11.1%	18.2%		50.0%	28.6%		28.6%	25.0%		50.0%	16.7%
pS1-B2 (aa 72-78 of pre-S1)	50.0%	0		44.4%	54.5%		37.5%	57.1%		50.0%	25.0%		30.0%	58.3%
pS1-BT (aa 94-117 of pre-S1)	50.0%	0		88.9%	81.8%		62.5%	64.3%		71.4%	25.0%		70.0%	25.0%	0.084
pS2-B1 (aa 1-6 of pre-S2)	0	0		88.9%	45.5%	0.070	50.0%	42.9%		64.3%	50.0%		100.0%	25.0%	< 0.001
pS2-B2 (aa 3-15 of pre-S2)	0	100.0%		88.9%	54.5%		62.5%	64.3%		71.4%	75.0%		100.0%	58.3%	0.040
pS2-B3 (aa 13-24 of pre-S2)	0	100.0%		44.4%	45.5%		37.5%	64.3%		35.7%	50.0%		70.0%	50.0%
pS2-T1 (aa 21-48 of pre-S2)	0	100.0%		33.3%	36.4%		25.0%	57.1%		14.3%	50.0%		40.0%	41.7%
Functional mapping
The start codon of L	0	50.0%		11.1%	9.1%		12.5%	42.9%		0	25.0%		20.0%	0
HBS (aa 2-48)	50.0%	50.0%		22.2%	18.2%		50.0%	50.0%		21.4%	50.0%		60.0%	25.0%
S promoter (nt 3045-3189)	50.0%	0		88.9%	81.8%		75.0%	71.4%		64.3%	75.0%		70.0%	66.7%
CBF (nt 3137-3147)	50.0%	0		66.7%	54.5%		50.0%	42.9%		57.1%	0	0.092	40.0%	25.0%
HSC70 (aa 74-118)	50.0%	0		88.9%	81.8%		62.5%	71.4%		71.4%	50.0%		70.0%	66.7%
CAD (aa 81-105)	50.0%	0		88.9%	81.8%		62.5%	64.3%		64.3%	50.0%		70.0%	25.0%	0.084
NBS (aa 103-127)	50.0%	0		100.0%	90.9%		75.0%	64.3%		92.9%	50.0%		100.0%	50.0%	0.015
The start codon of M	0	0		77.8%	54.5%		50.0%	50.0%		71.4%	50.0%		90.0%	25.0%	0.004
VS (aa 1-5 of pre-S2)	0	0		88.9%	45.5%	0.070	50.0%	42.9%		71.4%	50.0%		100.0%	25.0%	< 0.001
pHSA (aa 3-16 of pre-S2)	0	100.0%		88.9%	36.4%	0.028	62.5%	64.3%		78.6%	75.0%		100.0%	58.3%	0.040

¹P < 0.1. ASC: Asymptomatic carriers; CH: Chronic hepatitis; HBV: Hepatitis B virus; LC: Liver cirrhosis; LC-HCC: Liver cirrhotic hepatocellular carcinoma; NC-HCC: Noncirrhotic hepatocellular carcinoma; HBS: Hepatocyte binding site; CBF: CCAAT binding factor; HSC70: Heat shock protein 70; CAD: Cytosolic anchorage determinant; NBS: Nucleocapsid binding site; VS: Viral secretion; pHSA: Polymerized human serum albumin.