Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Sep 21, 2016; 22(35): 8026-8040
Published online Sep 21, 2016. doi: 10.3748/wjg.v22.i35.8026
Table 1 Primer and probe sequences used for real time qPCR
Gene nameProbe/primerSequence
IL-6Probe5-FAM ATTGCCATTGCACAACT-3
Forward5-GGGAAATCGTGGAAATGAGAAA-3
Reverse5-AAGTGCATCATCGTTGTTCATACA-3
MCP1Probe5-FAM-AATGGGTCCAGACATAC-3
Forward5-GTCTGTGCTGACCCCAAGAAG-3
Reverse5-TGGTTCCGATCCAGGTTTTTA-3
TNFαProbe5-FAM-TCACCCACACCGTCAG-3
Forward5-GGCTGCCCCGACTACGT-3
Reverse5-TTTCTCCTGGTATGAGATAGCAAATC-3
RAGEProbe5-FAM-CACAGCCCGGATTG-3
Forward5-GCTGTAGCTGGTGGTCAGAACA-3
Reverse5-CCCCTTACAGCTTAGCACAAGTG-3
alphaSMAProbe5-FAM-TGCCAGATCTTTTCC-3
Forward5-GACGCTGAAGTATCCGATAGAACA-3
Reverse5-GGCCACACGAAGCTCGTTAT-3
COL1A1Probe5-FAM-ATCGACCCTAACCAAG-3
Forward5-GACTGGAAGAGCGGAGAGTACTG-3
Reverse5-CCTTGATGGCGTCCAGGTT-3
CTGFProbe5-FAM-C ACTGCCTGGTCCAGAC-3
Forward5-GCTGCCTACCGACTGGAAGA-3
Reverse5-CTTAGAACAGGCGCTCCACTCT-3