Haemoxygenase modulates cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury

Figure 13 Increased Haemoxygenase expression was seen in all groups + ischemia reperfusion-24 as compared to ischemia reperfusion-24. A: Western blot analysis for HO in early and late phase of hepatic IR. Lane 1: Sham; Lane 2: MW- 32 Kda for HO protein; Lane 3: IR-24 (HO expression in late phase of hepatic IR) Lane 4-RS; Lane 5: RIPC + IR-24 (effect of RIPC on HO expression in late phase of IR); Lane 6: PDTC- HO inducer; Lane 7: Positive control; Lane 8: ZnPP- HO inhibitor; Lane 9: IR (early phase); B: Densitometry HO expression early phase. Early phase of hepatic IR (above). Densitometry for western blots showing increased HO-1 expression in RIPC + IR and PDTC + IR. Prior inhibition of HO (ZnPP + RIPC IR) significantly reduced HO expression in preconditioned animals; C: Densitometry Ho expression late phase. Densitometry for late phase of hepatic IR and RIPC western blot showing increased HO-1 expression in the RIPC group at 24 h. HO: Haemoxygenase; IR: Ischemia reperfusion.