Protein and gene expression characteristics of heterogeneous nuclear ribonucleoprotein H1 in esophageal squamous cell carcinoma

Figure 1 Expression and localization of heterogeneous nuclear ribonucleoprotein H1 in esophageal squamous cell carcinoma cells. A: Protein levels of HNRNPH1 were assessed by Western blots in seven ESCC cell lines. The β-actin protein was used as a loading control; B: Immunofluorescent visualization of HNRNPH1 in EC0156 cells; C: Subcellular protein levels of HNRNPH1 in EC0156 were assessed by western blots. Lamin B is a specific marker for nuclear proteins. AIF is a marker for intracellular membrane and nuclear proteins. Coomassie blue-stained SDS-PAGE gel was used as the loading control. AIF: Apoptosis inducing factor; HNRNPH1: Heterogeneous nuclear ribonucleoprotein H1; ESCC: Esophageal squamous cell carcinoma; SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis.